WIT Press

Modelling Surface Mediated Enzymatic Reactions In A Flow System*

Price

Free (open access)

Paper DOI

10.2495/BIO950271

Volume

2

Pages

8

Published

1995

Size

879 kb

Author(s)

R.D. Gentry & L. Ye

Abstract

The principal reaction initiating blood coagulation is the activation of fac- tor X by a tissue factor-factor Vila enzyme complex present on the surface of endothelium. To study this reaction a stochastic model was developed that simulates the diffusion and convection of substrate in a tubular flow system. A macroscopic kinetic model describes the activation of substrate reaching enzyme attached to the tube surface. The system is assumed to be radially symmetric and diffusion of substrate is modeled as a random walk on a rectangular grid representing the fluid and tube surface. Since diffusion of factor X on lipids is three orders of magnitude less than in fluids, surface diffusion is achieved by a delayed random walk. Simulations of reactions on tubes of lengths L < 0.01 cm generated steady state veloci- ties for different enzyme

Keywords