WIT Press

Bioremediation Of Land Oil Spills: Diversity Of Microorganisms Degrading Oil Hydrocarbons


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WIT Press


A. M. Boronin, A. E. Filonov, I. A. Kosheleva, A. N. Shkidchenko, A. B. Gafarov. S. L. Sokolov, I. F. Puntus, V. G. Grischenkov, V. V. Dmitriev & M. U. Arinbasarov


Bioremediation of land oil spills: diversity of microorganisms degrading oil hydrocarbons A.M. Boronin1, 2, A.E. Filonov1, 2, I.A. Kosheleva1, 2, A.N. Shkidchenko1, 2, A.B. Gafarov1, S.L. Sokolov1, I.F. Puntus1, V.G. Grishchenkov1, V.V. Dmitriev1 & M.U. Arinbasarov1. 1 Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences, Russia. 2 Pushchino State University, Russia. Abstract Environmental pollution by petroleum is an increasing global problem. In Russia this environmental problem is of such a large scale that it renders the excavation and off-site treatment of oil-contaminated soils and industrial wastes hardly possible. Therefore, in situ methods are attractive alternative. Bioremediation is a cost-effective and contaminant-specific approach. At the Institute of Biochemistry and Physiology of Microorganisms (Pushchino, Russia) there is a large collection of microorganisms degrading oil products. Their survival, stability, competitiveness, biodegradative activity, and efficiency under varying conditions have been investigated. Polycyclic aromatic hydrocarbons (PAH) belong to a recalcitrant group of environmental pollutants. They are components of oil and often occur in industrial wastes. The biochemical pathways of PAH catabolism and their genetic control in different strains were studied. It was shown that different combinations of host bacteria and plasmids changed growth characteristics and competitiveness of Psedomonas strains. The transfer of degradative plasmids may enhance the biodegradative potential of indigenous microorganisms. Model systems were elaborated to determine the efficiency of PAH degradation [l]. All these approaches can be used for the development of biotechnologies for clean up of oil-contaminated soils.