Author(s)

Eduardo Monteiro Martins, Priscila Falcão De Sá Borba, Neemias Espindola Dos Santos, Paula Thaise Bermudez Dos Reis, Renata Simões Silveira, Israel Felzenszwalb,Elisa Raquel Anastácio Ferraz, Andreia Da Silva Fernandes, Ronald Da Silva Muniz, Izabela Batista De Souza Matos, Sérgio Machado Correa

Abstract

The BTEX group (benzene, toluene, ethyl benzene and xylene) are known for their potential toxic, mutagenic and carcinogenic effects, especially in an indoor occupational environment, where these substances dissipate with greater difficulty. Thus, the present work evaluated the concentrations, mutagenicity and cytotoxicity of the BTEX group in the indoor air of workshops involving painting and varnishing. Samples were collected using air pumps from the outside of the open environment workshops and from the inside of each of the three workshops. The chemical analyses were carried out using gas chromatograph with mass spectrometry. The mutagenic and cytotoxicity potentials were determined using the Salmonella/microsome and WST/LDH assays, respectively. The concentrations of each constituent of the BTEX group were below the limits established by the National Institute for Occupational Safety and Health and toluene presented the highest value. Moreover, these compounds did not induce mutagenic activity in the TA98 and TA100 Salmonella typhimurium strains either in the presence or in the absence of metabolization, and no cytotoxic effects were observed in the A549 human lung cells. These results may be related to the low BTEX values found in the occupational environment, as can be seen in some other studies. Nevertheless, at low concentrations, these compounds may cause toxicity by a pathway not investigated in this study or may have interacted with other non-monitored air constituents, reducing their toxicity. The present study sought to obtain more information and clarifications regarding occupational exposure to BTEX, contributing to the risk assessment of the workers exposed to these substances.

Keywords

BTEX, exposure, indoor, mutagenicity, solvent, toxicity.